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Merck - 353919-1KIT - Glutathione Peroxidase Assay Kit

  • MFR SKU: 353919-1KIT
  • ITEM #: 6310817
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ITEM #: 6310817
Glutathione Peroxidase Assay Kit
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Merck - 353919-1KIT - Glutathione Peroxidase Assay Kit

  • Item #: 6310817
  • MFR SKU: 353919-1KIT
  • Overview A sensitive spectrophotometric assay (340 nm) for the measurement of glutathione peroxidase (GPx) activity in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. Assay range: 50-344 nmol/min/ml. Contains sufficient reagents for 96 tests.
  • Catalogue Number 353919
  • Brand Family Calbiochem®
  • Materials Required but Not Delivered • A plate reader with a 340 nm filter
  • • Adjustable pipettors and a repeat pipettor
  • • A source of pure water. Glass distilled water or HPLC-grade water is acceptable
  • • PBS
  • • Homogenization buffer (cell and tissue preparation)
  • References Ursini, F., et al. 1985. Biochim. Biophys. Acta 839, 62.
  • Forstrom, J.W., et al. 1978. Biochem. 17, 2639.
  • Paglia, D.E., et al. 1967. J. Lab. Clin. Med. 70, 158.
  • Detection method Colorimetric
  • Form 96 Tests
  • Format 96-well plate
  • Kit contains Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, NADPH, 96 Well Plate, Plate Cover, and a user protocol.
  • Positive control Bovine erythrocyte glutathione peroxidase
  • Quality Level MQ100
  • Assay range 50-344 nmol/min/ml
  • Assay time 2 h
  • Sample Type Cells, tissues, plasma
  • Ship Code Blue Ice Only
  • Toxicity Standard Handling
  • Storage -20° C
  • Storage Conditions Upon arrival store the entire contents of the kit at -20° C.
  • Do not freeze Ok to freeze
  • Kit contains Assay Buffer, Sample Buffer, Glutathione Peroxidase (Control), Co-Substrate Mixture, Cumene Hydroperoxide, NADPH, 96 Well Plate, Plate Cover, and a user protocol.

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