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Merck - 354103-1KIT - GLUTATHIONE ASSAY KIT II-1KIT (Each)

  • MFR SKU: 354103-1KIT
  • ITEM #: 6310819
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ITEM #: 6310819
Glutathione Assay Kit II^ Kit contains 2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.Format 96-well plate Form 96 Tests.Storage +2C to +8C
This listing is for Each

Merck - 354103-1KIT - GLUTATHIONE ASSAY KIT II-1KIT (Each)

  • Item #: 6310819
  • MFR SKU: 354103-1KIT
  • Overview A sensitive and convenient colorimetric (414 or 405 nm) assay kit for the measurement of glutathione from plasma, serum, erythrocyte lysate, tissue samples, and cultured cells. Dynamic range: 0 - 16 µ M for GSH or 0 - 8 µ M for GSSG. For plasma and serum samples concentration and deproteination are required.
  • Catalogue Number 354103
  • Brand Family Calbiochem®
  • Application Data
  • Materials Required but Not Delivered • A plate reader with a 414 or 405 nm filter.
  • • An adjustable pipettor, repeat pipettor, and an eight-channel pipettor (optional).
  • • A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
  • • Metaphosphoric acid and triethanolamine (described in Recommended procedure for deproteination of samples ).
  • • 2-vinylpyridine (optional, described in Sample preparation for exclusive measurement of GSSG ).
  • References Foyer, C.H., et al. 1994. Physiol. Plant. 92, 696.
  • Baillie, T.A. and Slatter, J.G. 1991. Acc. Chem. Res. 24, 264.
  • Baker, M.A., et al. 1990. Anal. Biochem. 190, 360.
  • Inoue, M., et al. 1987. Journal of Protein Chemistry 6, 207.
  • Eyer, P. and Podhradsky, D. 1986. Anal. Biochem. 153, 57.
  • Inoue, M. 1985. in Renal Biochemistry. Kinne, R.K.H. editor. Elsevier Science Publishers B.V. London, 225.
  • Lash, L.H. and Jones, D.P. 1985. Arch. Biochem. Biophys. 240, 583.
  • Griffith, O.W. 1980. Anal. Biochem. 106, 207.
  • Wendel, A. and Cikryt, P. 1980. FEBS Lett. 120, 209.
  • Glutathione: Metabolism and function , Arias, I.M. and Jakoby, W.B. 1976. editors. Raven Press, New York.
  • Tietze, F. 1969. Anal. Biochem. 27, 502.
  • Detection method Colorimetric
  • Form 96 Tests
  • Format 96-well plate
  • Kit contains 2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.
  • Positive control GSSG
  • Quality Level MQ100
  • Assay range 0 - 16 µ M for GSH or 0 - 8 µ M for GSSG
  • Assay time 3 h
  • Sample Type Plasma, serum, erythrocyte lysate, tissue samples, and cultured cells
  • Ship Code Blue Ice Only
  • Toxicity Standard Handling
  • Storage +2° C to +8° C
  • Storage Conditions Upon arrival store entire kit contents at 4° C.
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
  • Kit contains 2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.

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