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Merck - 391334-100GM - BES, Free Acid, ULTROL Grade | CAS 10191-18-1 | Calbiochem | 100GM

  • MFR SKU: 391334-100GM
  • ITEM #: 5882627
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ITEM #: 5882627
BES, Free Acid, ULTROL Grade^ ( [N, N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic acid]). White solid. BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.
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Merck - 391334-100GM - BES, Free Acid, ULTROL Grade | CAS 10191-18-1 | Calbiochem | 100GM

  • Item #: 5882627
  • MFR SKU: 391334-100GM
  • Overview BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
  • Absorbance (100 mM, 260 nm):≤ 0.05.
  • Catalogue Number 391334
  • Brand Family Calbiochem®
  • Synonyms N, N- bis -(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
  • References De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
  • Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
  • CAS number 10191-18-1
  • Form White solid
  • Hill Formula CHNOS
  • Chemical formula CHNOS
  • PKA 7.12 at 20° C
  • Quality Level MQ100
  • Purity ≥ 99% by titration (dry basis)
  • Heavy metals ≤ 1 ppm
  • RTECS KI7363500
  • Ship Code Ambient Temperature Only
  • Toxicity Standard Handling
  • Storage +15° C to +30° C
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, store in the refrigerator (4° C). Stock solutions are stable for up to 2 weeks at 4° C.

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