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Merck - 420200-5MG - JC-1 | CAS 47729-63-5 | Calbiochem | 5MG

  • MFR SKU: 420200-5MG
  • ITEM #: 6280893
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ITEM #: 6280893
JC-1^ 5mg. 5, 5', 6, 6'-Tetrachloro-1, 1', 3, 3'-tetraethylbenzimidazolylcarbocyanine Iodide. Red-orange solid. Cationic fluorescent carbocyanine mitochondrial-sensitive dye. Mitochondrial depolarization is indicated by decrease in red/green fluorescence intensity ratio. Purity =95% TLC. CAS: 47729-63-5.
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Merck - 420200-5MG - JC-1 | CAS 47729-63-5 | Calbiochem | 5MG

  • Item #: 6280893
  • MFR SKU: 420200-5MG
  • Overview A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ m in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨ m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
  • Catalogue Number 420200
  • Brand Family Calbiochem®
  • Synonyms 5, 5, 6, 6-Tetrachloro-1, 1, 3, 3-tetraethylbenzimidazolylcarbocyanine Iodide
  • References Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
  • Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
  • Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
  • Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
  • Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
  • White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
  • Ankacrona, M., et al. 1995. Neuron 15, 961.
  • Reers, M., et al. 1995. Methods Enzymol. 260, 406.
  • Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
  • CAS number 47729-63-5
  • ATP Competitive N
  • Form Red-orange solid
  • Hill Formula CHClIN
  • Chemical formula CHClIN
  • Reversible N
  • Structure formula Image
  • Quality Level MQ100
  • Primary Target Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria
  • Purity ≥ 95% by HPLC
  • Cell permeable N
  • R Phrase R: 36/37/38
  • Irritating to eyes, respiratory system and skin.
  • S Phrase S: 26-36
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Wear suitable protective clothing.
  • Ship Code Ambient Temperature Only
  • Toxicity Irritant
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot and freeze (-20° C). Stock solutions are stable for up to 6 months at -20° C. Do not reuse thawed samples.
  • Packaged under inert gas Packaged under inert gas

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