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Merck - 437639-1KIT - Lipid Hydroperoxide (LPO) Assay Kit

Name: Merck - 437639-1KIT - Lipid Hydroperoxide (LPO) Assay Kit
SKU: 437639-1KIT
Price: 1127.0 USD
Availability: InStock

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ITEM #: 6310941
Lipid Hydroperoxide (LPO) Assay Kit
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Overview A sensitive and reliable assay kit for the measurement of the hydroperoxides from any sample containing lipid hydroperoxides, directly utilizing the redox reactions with ferrous ions. Quantification of lipid peroxidation is essential to assess the role of oxidative injury in pathophysiological disorders.
Catalogue Number 437639
Brand Family Calbiochem®
Materials Required but Not Delivered • A plate reader or spectrophotometer with a 500 nm filter
• An adjustable pipettor
• A source of pure water. Glass distilled water or HPLC-grade water is acceptable
• Deoxygenated chloroform and methanol (see Reagent Preparation)
• A reusable glass 96 well plate
References Morrow, J.D. and Roberts, L.J. 1997. Prog. Lipid Res. 36, 1.
Halliwell, B. 1996. Free Radic. Res. 25, 57.
Hoffman, S.W., et al. 1996. J. Neurosci. Methods 68, 133.
Mihaljevic, B., et al. 1996. Free Radic. Biol. Med. 21, 53.
Porter, N.A., et al. 1995. Lipids 30, 277.
Esterbauer, H., et al. 1991. Free Radic. Biol. Med. 11, 81.
Janero, D.R., 1990. Free Radic. Biol. Med. 9, 515.
Pryor, W.A., et al. 1990. Free Radic. Biol. Med. 8, 541.
Cross, C.E., et al. 1987. Ann. Intern. Med. 107, 526.
Yamamoto, Y., et al. 1987. Anal. Biochem. 160, 7.
Warso, M.A. and Lands, W.E.M. 1984. Clin. Physiol. Biochem. 2, 70.
Diczfalusy, U., et al. 1977. FEBS Lett. 84, 271.
Bligh, E.G. and Dyer, W.J. 1959. Can. J. Biochem. Physiol. 37, 911.

Detection method Colorimetric
Form 100 Tests
Format Cuvette or 96-well plate
Kit contains FTS Reagent 1, FTS Reagent 2, Lipid Hydroperoxide Standard, Extract R, Triphenylphosphine, and a user protocol.
Positive control 13-hydroperoxyoctadecanoic acid
Quality Level MQ100
Assay range 0.25-5 nmol hydroperoxide per assay tube
Assay time 1.5 h
Sample Type Tissues, cultured cells, plant materials, foods, biological fluids, etc.
Intended use The Calbiochem ® Lipid Hydroperoxide (LPO) Assay Kit is designed to measure lipid hydroperoxides in any sample type containing detectable levels (e.g. cells, tissues, plant material, biological fluids).
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2° C to +8° C
Storage Conditions Upon arrival store the Lipid Hydroperoxide Standard at -80° C. The remaining components of the kit should be stored at 4° C.
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Kit contains FTS Reagent 1, FTS Reagent 2, Lipid Hydroperoxide Standard, Extract R, Triphenylphosphine, and a user protocol.

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