Merck - 4655885001 - Transcriptor One-Step RT-PCR Kit, 150rx

ITEM #: 13425445
For hot start one-step RT-PCR, utilizing the enzyme mi x ture of Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the E x pand System, with an innovative hot start buffer (patents pending).
One-step RT-PCR is a very sensitive technique for determining the presence or absence of RNA templates, or to quantify the levels of e x pression through qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels (e.g., for virus-level quantification). This RT-PCR method also facilitates the amplification of rare messages for cloning. The one-step format is convenient, shows reduced reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.
The Transcriptor One-Step RT-PCR Kit offers the outstanding benefits of Transcriptor Reverse Transcriptase, as well as the advantages of a hot start one-step RT-PCR format.
The Transcriptor One-Step RT-PCR Kit provides all components required for one-step RT-PCR (e x cept primers and template) in a convenient format. The 50-reaction pack size also includes 10 control reactions (control RNA and control primer mi x ).
The kit′s RT-PCR Enzyme Mi x contains four different enzymes: Transcriptor Reverse Transcriptase ensures sensitive and robust reverse transcription with high yield; Protector RNase Inhibitor, fully active at elevated temperatures, provides ma x imum template protection during reverse transcription; and the E x pand System - a blend consisting of Taq DNA Polymerase and a proofreading polymerase - minimizes the possibility of mutations, offering high yield and fidelity in the PCR.
The optimized RT-PCR Reaction Buffer includes high quality dNTPs and provides the overall improved performance of a hot start system. The buffer′s unique hot start component binds and sequesters primers at lower temperatures to prevent the primers from binding to nonspecific sites. Another component binds magnesium, in a temperature-dependent manner, to prevent uncontrolled DNA synthesis. This new buffer formulation is effective during reverse transcription as well as PCR, resulting in increased specificity and sensitivity.
The combination of optimized reaction buffer and enzyme mi x ensures efficient transcription of difficult templates with high secondary structure and GC content without increasing reaction temperature. Features and Benefits
• Obtain high sensitivity and yield in a wide amplification range: Detect as little as 1 fg of total RNA
• Achieve high specificity and reduce primer-dimers : Count on e x cellent performance with the kit’s proprietary hot start buffer (patents pending)
• Produce long fragments: Generate transcripts up to 6.5 kb.
• Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA) : Overcome high secondary structure with thermostableTranscriptor Reverse Transcriptase in combination with an optimized buffer.
• Save time and improve results with a convenient kit: Minimize pipetting steps and prevent contamination by using a premi x ed enzyme blend and optimized buffer that includes PCR-grade dNTPs and hot start components.
• Protect your RNA sample: Safeguard your RNA from degradation by RNases with the supplied thermostable Protector RNase Inhibitor that is functional during one-step RT-PCR when other RNase inhibitors fail
• Increase confidence in your results : Use the supplied control reactions to monitor performance
Transcriptor One-Step RT-PCR Kit, 150rx
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