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Merck - 539576-25UG - PKA, Catalytic Subunit, Bovine Heart

  • MFR SKU: 539576-25UG
  • ITEM #: 6311018
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ITEM #: 6311018
Protein Kinase A Catalytic Subunit^ cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit. Form: Liquid, purity: =95% by SDS-PAGE, Storage: = -70DegreeC 25ug
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Merck - 539576-25UG - PKA, Catalytic Subunit, Bovine Heart

  • Item #: 6311018
  • MFR SKU: 539576-25UG
  • Overview Native PKA catalytic subunit purified from bovine heart. The catalytic subunit of PKA dissociates from the PKA holoenzyme following binding of cAMP to the PKA regulatory subunits and phosphorylates target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit does not require cAMP for activation and is suitable for studies of PKA phosphorylation in vitro with purified substrates or following its introduction into intact cells or cell extracts.
  • Note: 25 µg ≥ 500, 000 units.
  • Catalogue Number 539576
  • Brand Family Calbiochem®
  • Synonyms cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
  • Application Data
  • References Corbin, J.D., et al. 1988. Methods Enzymol 159, 74.
  • Rannels, S.R., et al 1983. Methods Enzymol 99, 55.
  • Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem. 66, 253.
  • Unit of Definition One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30° C.
  • Form Liquid
  • Formulation In 350 mM KHPO, 100 µ M DTT, pH 6.8.
  • Quality Level MQ100
  • Purity ≥ 90% by SDS-PAGE
  • Specific Activity ≥ 20, 000 units/µg protein
  • Concentration Label Please refer to vial label for lot-specific concentration
  • Ship Code Dry Ice Only
  • Toxicity Standard Handling
  • Storage ≤ -70° C
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
  • Special Instructions Following initial thaw, aliquot and freeze (-70° C).

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