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Merck - 566660-5MG - Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem

  • MFR SKU: 566660-5MG
  • ITEM #: 11575937
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ITEM #: 11575937
A cell-permeable Smoothened Agonist, SAG, CAS 364590-63-6, modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K D = 59 nM).
This listing is for 5 mg Glass bottle

Merck - 566660-5MG - Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem

  • Item #: 11575937
  • MFR SKU: 566660-5MG
  • Overview A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (K D = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC 50 = ∼ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804 ) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661 ) in H 2 O is also available.
  • Catalogue Number 566660
  • Brand Family Calbiochem®
  • Synonyms N-Methyl-N-(3-pyridinylbenzyl)-N-(3-chlorobenzo[b]thiophene-2-carbonyl)-1, 4-diaminocyclohexane, SAG 1.3
  • References Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
  • Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
  • Chen, W., et al. 2004. Science 306, 2257.
  • Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
  • Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
  • CAS number 364590-63-6
  • Form Light yellow solid
  • Hill Formula CHClNOS • 2HCl • 2HO
  • Chemical formula CHClNOS • 2HCl • 2HO
  • Hygroscopic Hygroscopic
  • Structure formula Image
  • Quality Level MQ100
  • Purity ≥ 98% by HPLC
  • Ship Code Ambient Temperature Only
  • Toxicity Standard Handling
  • Storage +2° C to +8° C
  • Protect from Light Protect from light
  • Hygroscopic Hygroscopic
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot and freeze (-20° C). Stock solutions are stable for up to 6 months at -20° C.
  • Packaged under inert gas Packaged under inert gas

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