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Merck - 569393-500UG - Stauprimide (ESC EMT Inducer) | CAS 154589-96-5 | Calbiochem | 500 UG

  • MFR SKU: 569393-500UG
  • ITEM #: 3476510
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ITEM #: 3476510
A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differentiation into progenitor cells of multiple lineages.
This listing is for 500 μg

Merck - 569393-500UG - Stauprimide (ESC EMT Inducer) | CAS 154589-96-5 | Calbiochem | 500 UG

  • Item #: 3476510
  • MFR SKU: 569393-500UG
  • Overview A cell-permeable ESCs (Embryonic Stem Cells) EMT (Epithelial-Mesenchymal Transition) inducer that is reported to prime/sensitize human and murine ESC cultures for much more enhanced/efficient differentiation into progenitor cells of multiple lineages, including Activin A- (Cat. No. 114700 ) induced definitive endoderm differentiation (60% differentiation from murine R1 ESCs with 5 ng/ml Activin A and 200 nM Spd), ATRA- (Cat. No. 554720 ) induced neuronal progenitor differentiation, and BMP-4- (Cat. No. 203642 ) induced mesoderm differentiation, under suboptimal lineage specifying stimuli concentrations and often serum-free conditions, while Spd sensitization alone does not induce any ESCs differentiation. Spd is shown to interact with NME2/DNPK B/PUF and prevent NME2 nuclear localization and c-myc transcription in murine R1 ESC cultures (46% and 85% drop in c-myc mRNA in 24 h, respectively, with 200 nM and 500 nM Spd), which accounts for, at least in part, its EMT induction activity. Although a structural analog of the broad-spectrum kinase inhibitors Staurosporine (Cat. Nos. 569396 and 569397 ) and UCN-01 (Cat. No. 539644 ), Spd exhibits significant kinase inhibitory activities only at elevated concentrations, but not at concentrations ≤ 500 nM typically used in EMT induction.
  • Catalogue Number 569393
  • Brand Family Calbiochem®
  • Synonyms ESC EMT Inducer, Spd
  • References Zhu, S., et al. 2009. Cell Stem Cell 4, 416.
  • CAS number 154589-96-5
  • Form Yellow solid
  • Hill Formula CHNO
  • Chemical formula CHNO
  • Structure formula Image
  • Quality Level MQ100
  • Purity ≥ 95% by HPLC
  • S Phrase S: 22-24/25
  • Do not breathe dust.
  • Avoid contact with skin and eyes.
  • Ship Code Shipped with Blue Ice or with Dry Ice
  • Toxicity Standard Handling
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliqout and freeze (-70° C). Stock solutions are stable for up to 6 months at -70° C.
  • Packaged under inert gas Packaged under inert gas

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