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Merck - 574601-1KIT - SUPEROXIDE DISMUTASE ASSAY KIT (Each)

  • MFR SKU: 574601-1KIT
  • ITEM #: 6311059
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ITEM #: 6311059
Superoxide Dismutase Assay Kit II^ Format: 96-well plate, Form: 96 Tests. It includes Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
This listing is for Each

Merck - 574601-1KIT - SUPEROXIDE DISMUTASE ASSAY KIT (Each)

  • Item #: 6311059
  • MFR SKU: 574601-1KIT
  • Overview A sensitive spectrophotometric assay (450 nm) for the measurement of all three types (Cu/Zn, Mn, and Fe) of SOD.
  • Catalogue Number 574601
  • Brand Family Calbiochem®
  • Materials Required but Not Delivered • Plate reader capable of reading absorbance at 450 nm.
  • • Adjustable pipettors and a repeat pipettor.
  • • A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
  • References Maier, C.M. and Chan, P.H 2002. The Neuroscientist 8, 323.
  • Mattiazz, M., et al. 2002. J. Biol. Chem. 277, 29626.
  • Beckman, J.S. and Koppenol, W.H. 1996. Am. J. Physiol. 271, C1424.
  • Liu, D. 1996. J. Mol. Neuro. 7, 159.
  • MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.
  • Sun, E., et al. 1995. Biol. Trace Elem. Res. 48, 231.
  • Sandstrom, J., et al. 1994. J. Biol. Chem. 269, 19163.
  • Marklund, S. 1980. Acta Physiol. Scand. Suppl. 492, 19.
  • Malstrom, B. 1975. in The Enzymes. Boyer, P., editor. XIIB, Academic Press, New York, 533.
  • Unit of Definition The amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.
  • Detection method Colorimetric
  • Form 96 Tests
  • Format 96-well plate
  • Kit contains Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
  • Positive control Bovine erythrocyte SOD (Cu/Zn)
  • Quality Level MQ100
  • Assay range 0.025-0.25 units per ml SOD
  • Assay time 1.5 h
  • Sample Type Plasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates
  • Ship Code Dry Ice Only
  • Toxicity Standard Handling
  • Storage -20° C
  • Storage Conditions Upon arrival store entire contents of the kit at -20° C.
  • Do not freeze Ok to freeze
  • Kit contains Assay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.

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