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Merck - 5.00527.0001 - APETx2, Anthopleura elegantissima, Recombinant, E. coli - Calbiochem

  • MFR SKU: 5.00527.0001
  • ITEM #: 13025357
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$660.50
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ITEM #: 13025357
This listing is for 100 μg Glass bottle

Merck - 5.00527.0001 - APETx2, <i>Anthopleura elegantissima</i>, Recombinant, <i>E. coli</i> - Calbiochem

  • Item #: 13025357
  • MFR SKU: 5.00527.0001
  • Overview Originally isolated from sea anemone Anthopleura elegantissima , the 42 amino-acid toxin APETx2 is shown to block acidification-induced activation of homotrimeric acid-sensing ion channel composed of rat or human ASIC3, but not rat ASIC1a, ASIC1b, or ASIC2a, in a reversible manner. APETx2 is also shown to inhibit the Na + -dependent, alkalization-induced "nonconventional" channel activity seen in human, but not rat, ASIC3. APETx2 in vivo efficacy is demonstrated in various rat pain induction models (100 µ L 2.2 µ M APETx2 via i.m. injection). Reported to display much reduced potency against Kv3.4 and little or no effect toward hERG, Kv2.2, Kv3.1, Kv4.2, or Kv4.3 channel activity.
  • Catalogue Number 500527
  • Brand Family Calbiochem®
  • Synonyms Acid-Sensing Ion Channel 3 Blocker, ASIC3 Channel Blocker
  • References Delaunay, A., et al. 2012. Proc. Natl. Acad. Sci. 109, 13124.
  • Sherwood, T.W., et al. 2012. Am. J. Physiol. Cell Physiol. 303, C699.
  • Karczewski, J., et al. 2010. Br J Pharmacol. 161, 950.
  • Chagot, B., et al. 2005. Protein Sci. 14, 2003.
  • Diochot, S., et al. 2004. EMBO J. 23, 1516.
  • Form Lyophilized powder
  • Formulation Supplied as a trifluoroacetate salt.
  • Hill Formula CHNOS
  • Chemical formula CHNOS
  • Reversible Y
  • Quality Level MQ100
  • Primary Target ASIC3 50 $anchor_PDP_OverviewTab_Product_Biological_Info_Primary Target IC 50 " value=" Biological Information"
  • br> Primary Target IC<sub> 50</sub> 63 nM
  • Purity ≥ 95% by HPLC
  • Peptide Sequence H-Gly-Thr-Ala-Cys-Ser-Cys-Gly-Asn-Ser-Lys-Gly-Ile-Tyr-Trp-Phe-Tyr-Arg-Pro-Ser-Cys²-Pro-Thr-Asp-Arg-Gly-Tyr-Thr-Gly-Ser-Cys³-Arg-Tyr-Phe-Leu-Gly-Thr-Cys³-Cys³-Thr-Pro-Ala-Asp-OH (disulfide bonds: 4 → 37, 6 → 30, 20 → 38)
  • Ship Code Shipped with Blue Ice or with Dry Ice
  • Toxicity Standard Handling
  • Storage -20° C
  • Protect from Light Protect from light
  • Do not freeze Ok to freeze
  • Special Instructions Following reconstitution, aliquot and freeze (-20° C). Stock solutions are stable for up to 3 months at -20° C.
  • Packaged under inert gas Packaged under inert gas

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