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Merck - 69053-4 - BLR(DE3) Competent Cells - Novagen

  • MFR SKU: 69053-4
  • ITEM #: 3440948
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ITEM #: 3440948
BLR(DE3), pET Host Strain Competent Cells^ pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).
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Merck - 69053-4 - BLR(DE3) Competent Cells - Novagen

  • Item #: 3440948
  • MFR SKU: 69053-4
  • Overview BLR is a recA - derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).
  • DE3 indicates that the host is a lysogen of λ DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
  • Genotype: F - ompT hsdS B (r B - m B - ) gal lac ile dcm Δ( srl-recA ) 2 x 10 cfu/μg
  • Quality Level MQ100
  • Ship Code Dry Ice Only
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage ≤ -70° C
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze

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