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Merck - 69831-3 - MOUSE IG-PRIMER SET 3 (Each)

  • MFR SKU: 69831-3
  • ITEM #: 6311182
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ITEM #: 6311182
Mouse Ig-Primer Set 3^ Components: Mu IgM VH 3-1 Primer, Mu IgG VH 3-2 Primer, Mu Ig VH 5-A Primer, Mu Ig VH 5-B Primer, Mu Ig VH 5-C Primer, Mu Ig VH 5-D Primer, Mu Ig VH 5-E Primer, Mu Ig VH 5-F Primer, Mu Ig-+ VL 3-1 Primer, Mu Ig-+ VL 5-A Primer, Mu Ig-+ VL 5-B Primer
This listing is for Each

Merck - 69831-3 - MOUSE IG-PRIMER SET 3 (Each)

  • Item #: 6311182
  • MFR SKU: 69831-3
Technical Specs
  • Overview The Ig-Primer Sets are designed for amplification of immunoglobulin (Ig) variable region cDNAs from human and mouse sources. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR (Larrick 1989, Jones 1991), after which the products may be sequenced directly or cloned. This powerful method enables rapid, precise analysis of hybridomas, myeloma cell progression and heterogeneity, idiotype networks, B-cell clonal ontogeny, and phylogenetic relationships.
  • In practice, total RNA is isolated from a B-cell source, such as peripheral blood, lymph node(s), spleen, or hybridoma, and first-strand cDNA is prepared using reverse transcriptase. The primer sets are then used in PCR to specifically amplify the variable regions of light- and heavy-chain cDNAs.
  • Please call Technical Service if you need more information regarding individual Ig-Primer Sets.
  • Catalogue Number 69831
  • Brand Family Novagen®
  • References Larrick, J.W., et al. 1989. Biochem. Biophys. Res.
  • Comm. 160 , 1250. Jones, S.T. and Bendig, M.M. 1991.
  • Biotechnology 9 , 88.
  • • 500 pmol Mu IgM V H 3-1 Primer
  • • 500 pmol Mu IgG V H 3-2 Primer
  • • 500 pmol Mu Ig V H 5-A Primer
  • • 500 pmol Mu Ig V H 5-B Primer
  • • 100 pmol Mu Ig V H 5-C Primer
  • • 100 pmol Mu Ig V H 5-D Primer
  • • 100 pmol Mu Ig V H 5-E Primer
  • • 100 pmol Mu Ig V H 5-F Primer
  • • 500 pmol Mu Igκ V L 3-1 Primer
  • • 500 pmol Mu Igκ V L 5-A Primer
  • • 500 pmol Mu Igκ V L 5-B Primer
  • • 100 pmol Mu Igκ V L 5-C Primer
  • • 100 pmol Mu Igκ V L 5-D Primer
  • • 100 pmol Mu Igκ V L 5-E Primer
  • • 100 pmol Mu Igκ V L 5-F Primer
  • • 100 pmol Mu Igκ V L 5-G Primer
  • • 500 pmol Mu Igλ V L 3-1 Primer
  • • 500 pmol Mu IgMλ V L 5-A Primer
  • • 10 µl Mouse Positive Control PCR Template
  • Quality Level MQ100
  • Ship Code Shipped with Blue Ice or with Dry Ice
  • Toxicity Standard Handling
  • Storage -20° C
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
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