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Merck - 71353-4 - Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen

  • MFR SKU: 71353-4
  • ITEM #: 5010525
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ITEM #: 5010525
Rosetta-Gami 2 strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
This listing is for 1 ml Glass bottle

Merck - 71353-4 - Rosetta-gami 2(DE3)pLacI Competent Cells - Novagen

  • Item #: 5010525
  • MFR SKU: 71353-4
Technical Specs
  • Overview Expression host strains for pETBlue™ and pTri. Ex™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7 lac promoter in the absence of inducer. The Nova. Blue host strain is recommended for initial cloning with pETBlue and pTri. Ex vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.
  • Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors.
  • . Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E. coli . These trxB / gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs. In Rosetta-gami 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.
  • DE3 indicates that the host is a lysogen of λ DE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
  • Genotype: Δ( ara - leu ) 7697 Δ lacX74 Δ phoA Pvu II phoR araD139 ahpC galE galK rpsL (DE3) F′[ lac + lacI q pro ] gor522 ::Tn 10 trxB pLacIRARE2 (Cam R , Str R , Tet R )
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Millipore. Sigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@ .
  • Catalogue Number 71353
  • Brand Family Novagen®
  • 0.4 ml 1 ml Component
  • • 2 × 0.2 ml 5 × 0.2 ml Rosetta-gami 2(DE3)pLacI Competent Cells
  • • 2 × 2 ml 4 × 2 ml SOC Medium
  • • 2 ng 2 ng Test Plasmid
  • Guaranteed efficiency 2 × 10 cfu/µg
  • Quality Level MQ100
  • Ship Code Dry Ice Only
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage ≤ -70° C
  • Do not freeze Ok to freeze
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