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Merck - 71546-3 - BacMagic Transfection Kit - Novagen

  • MFR SKU: 71546-3
  • ITEM #: 6311403
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ITEM #: 6311403
BacMagic TRANSFECTION KIT 5RXN
BacMagic TRANSFECTION KIT
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Merck - 71546-3 - BacMagic Transfection Kit - Novagen

  • Item #: 6311403
  • MFR SKU: 71546-3
  • Overview The Bac. Magic™-1 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Bac. Magic DNA is an AcNPV genome with a portion of the essential open reading frame (ORF) 1629 deleted, preventing nonrecombinant virus from replicating in insect cells. The target coding sequence in a compatible transfer plasmid, such as pIEx/Bac™, pBAC™, or pTri. Ex™ vectors, is cotransfected with Bac. Magic DNA into insect cells. Homologous recombination within the cells restores the function of the viral ORF1629. Only recombinant baculovirus can replicate, producing a homogeneous population of recombinants. The Bac. Magic™-1 Transfection Kit contains the Bac. Magic™-1 DNA, Insect Gene. Juice ® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and Bac. Vector ® Medium, for optimal transfection performance.
  • Catalogue Number 71546
  • Brand Family Novagen®
  • Application Data
  • Figure 1. Construction of baculovirus recombinants with three Bac. Magic systems.
  • Figure 2. Streamlined baculovirus method
  • • 0.5 µg Bac. Magic DNA
  • • 50 µl Insect Gene. Juice Transfection Reagent
  • • 2 x 1 ml Sf9 Cells
  • • 1 L Bac. Vector ® Insect Cell Medium
  • • 2 µg Transfection Control Plasmid
  • Quality Level MQ100
  • Ship Code Multiple Storage Conditions
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage Multiple storage requirements
  • Do not freeze Ok to freeze

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