Merck - 71975-3 - KOD Xtreme Hot Start DNA Polymerase 200u

ITEM #: 6294720
The KOD Xtreme Hot Start DNA Polymerase* kit is an optimized PCR system for the amplification of long or GC-rich DNA templates. The unique formulation enables you to amplify directly from minimally processed samples; allowing highly efficient crude sample PCR target amplification. The system includes an ultra high fidelity KOD DNA polymerase comple x ed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively. KOD Xtreme Hot Start DNA Polymerase successfully amplifies challenging DNA templates with up to 90% GC content. Each kit provides 200 U KOD Xtreme Hot Start DNA Polymerase, an optimized buffer and dNTPs sufficient for 200 amplification reactions. The polymerase produces blunt-ended DNA products suitable for cloning with the Novagen Perfectly Blunt and LIC Vector Kits.
*Manufactured by Toyobo and distributed by EMD. Not available from Merck in Japan.
Features and Benefits:

• Optimized for the highest PCR success rate, even with the most difficult targets
• 10X higher fidelity than Taq blends
• Efficiently amplifies up to 90% GC-content DNA templates
• Amplifies genomic targets up to 24 kbp
• Amplifies phage/Lambda targets up to 40 kbp
• Eliminates mispriming and primer-dimer formation
• Convenient ambient-temperature setup compatible with automation

One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mi x of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA.
Components:

• 200 U KOD Xtreme Hot Start DNA Polymerase
• 1.7 ml 2X Xtreme Buffer
• 1 ml dNTPs (2 mM each)

KOD Xtreme Hot Start DNA Polymerase 200u
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