Merck - A2103-200UL - SIGMA Anti-Actin, N-terminal antibody produced in rabbit, 200µL

ITEM #: 13045259
Actin is one of the most conserved eukaryotic proteins, which is e x pressed in mammals and birds as at least si x major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.
MDL Number: MFCD00145889
Storage: -20C
Application: Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.
Biochem Physiol Actions: Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coe x ist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.
SIGMA Anti-Actin, N-terminal antibody produced in rabbit, 200µL
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