Merck - ABS1023 - ABS1023, Anti-Angiomotin Antibody from rabbit, purified by affinity chromatography

ITEM #: 11556382
Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemota x is, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein comple x associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is e x pressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.
Synonyms: Angiomotin
Application: Western Blotting Analysis: A representative lot detected phospho Angiomotin (Ser175) in HEK293 transfected with AMOT cell lysate (Dr. Joseph Kissil, Scripps Research Institute, Florida).Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells e x pressing equal amount of HA-Merlin and Myc-Rich1, untreated (-) or Do x ycyline induced (+) RT4-67 cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).Western Blotting Analysis: A representative lot detected Angiomotin in Flag-IP and total cell lysate from HEK293 cells transfected with Flag-tagged wild-type (WT) or patient-derived mutant Merlin alleles, HEK293, ERK activation in Nf2-/- SC4 cells or Merlin-reconstituted SC4 cells (Nf2-/-+retroNf2) (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).Western Blotting Analysis: A representative lot detected Angiomotin in HEK293, MEFs treated with Adeno (-) orAdeno-Cre (+) virus (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).Immunofluorescence Analysis: A representative lot detected Angiomotin in epithelial cells (Yi, C., et al. (2011) Cancer Cell. 19(4):527-540).Immunofluorescence Analysis: A representative lot detected Angiomotin in liver from Amot KO mice (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).Immunocytochemistry Analysis: A representative lot detected Angiomotin in SC4 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Angiomotin in HEK293 cells (Yi, C., et al. (2013) Cell Signaling. 6(291):1-12).
Other Notes: Concentration: Please refer to lot specific datasheet.
ABS1023, Anti-Angiomotin Antibody from rabbit, purified by affinity chromatography
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