Merck - ABS1640 - ABS1640, Anti-SPTLC3 Antibody from rabbit

ITEM #: 11556458
Serine palmitoyltransferase 3 (EC 2.3.1.50; UniProt Q9NUV7; also known as LCB 3, LCB2b, Long chain base biosynthesis protein 2b, Long chain base biosynthesis protein 3, Serine-palmitoyl-CoA transferase 3, SPT 3) is encoded by the SPTLC3 (also known as C20orf38, SPTLC2L) gene (Gene ID 55304) in human. Serine palmitoyltransferase (SPT) is a pyrido x al 5'-phosphate (PLP)-dependent enzyme that catalyses the first step of de novo sphingolipids (SLs) biosynthesis, namely the condensation of L-serine with palmitoyl coenzyme-A to generate 3-ketodehydrosphinganine (KDS) that forms the sphingoid base backbone of all SLs. The core human SPT homoenzyme is a membrane-bound heterodimer composed of an hLCB1 (SPTLC1) subunit together with either an hLCB2a (SPTLC2) or an hLCB2b (SPTLC3) subunit. The hLCB1/hLCB2 heterodimer contains a single active site composed of residues from both subunits. hLCB2a and hLCB2b share 68% sequence identity, with hLCB2b being the isotype that is predominantly e x pressed in the placenta and prefers shorter chain acyl-CoAs (C12 and C14) to generate short-chain SLs. In addition, two human small subunits (ssSPTa and ssSPTb) have been identified and found to ma x imize the catalytic activity and to confer the acyl-CoA substrate chain-length specificity of the hLCB1/hLCB2 core enzyme. Mutations in hLCB1 and hLCB2a are found in patients with hereditary sensory and autonomic neuropathy type I (HSAN1), an inherited disorder that affects sensory and autonomic neurons due to neuroto x ic deo x ysphingolipids formation as a result of substrate promiscuity of the mutated SPT enzyme.
Synonyms: Serine palmitoyltransferase 3, LCB 3, LCB2b, Long chain base biosynthesis protein 2b, Long chain base biosynthesis protein 3, Serine-palmitoyl-CoA transferase 3, SPT 3
Application: Western Blotting Analysis: The purified polyclonal antibody was used to detect the presence of SPTLC2 in SPTLC1 and SPTLC2 immunoprecipiates from human placenta tissue homogenate (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164).Western Blotting Analysis: The purified polyclonal antibody was used to detect the endogenous SPTLC3 in human cell lysates (HepG2, JAR, JEG3) and human placenta tissue homogenate, as well as e x ogenously e x pressed SPTLC3 in transfected HEK293 cells (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164; Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).
Other Notes: Concentration: Please refer to lot specific datasheet.
ABS1640, Anti-SPTLC3 Antibody from rabbit
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