Merck - ABS1670 - ABS1670, Anti-Phosphohistidine (pHis) from rabbit, purified by affinity chromatography

ITEM #: 11556472
Phosphorylation plays an important role in regulating protein activities and various cellular signaling events in cells. Limited by the tools available for phosphohistidine (p. His) detection, the majority of studies focus on serine, threonine, and tyrosine phosphorylations. Histidine phosphorylation can occur at either N1 (1-p. His) or N3 (3-p. His) of the imidazole ring. The development of stable phosphoryltriazolylalanine analogues of p. His (p. Tza and p. Pza) allows the generation of antibodies for studying both histidine N1 and N3 phosphorylations in signaling events. There is growing evidence implicating His kinases in cancer and tumor metastasis and the first metastasis suppressor gene identified is one of the two known mammalian His kinases, Nm23-H1 (also known as NME1, nucleoside diphosphate kinase, or NDPK-A). Nm23-H1/NME1 and the closely related Nm23-H2 (NME2/NDPK-B) catalyze the transfer of phosphate from ATP onto Nucleoside-diphosphates (NDPs) through a 1-p. His enzyme intermediate. Nm23-H1/-H2 also possess His kinase activity, transferring the phosphate from the active site p. His onto a His in a target protein. Metabolic enzymes such as phosphoglycerate mutase (PGAM), succinyl CoA synthase (SCS), and ATP citrate lyase (ACL) also use p. His as an enzyme intermediate. Unlike NME1/2, PGAM uses 3-p. His as an enzyme intermediate. In addition to eukaryotes, histidine phosphorylation is well documented in bacterial two-component signaling pathways involved in chemota x is, although the phosphate is transferred from the p. His formed in the receptor/sensor protein to Asp residues of an acceptor response regulator protein, and the receptor/sensor protein essentially functions as an aspartate kinase.
Synonyms: p. His, phosphohistidine
Application: Research Category. Signaling
Other Notes: Concentration: Please refer to lot specific datasheet.
ABS1670, Anti-Phosphohistidine (p. His) from rabbit, purified by affinity chromatography
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