Merck - AG4000 - uPA Colorimetric Substrate, ECM600, ECM610

ITEM #: 11557009
95% by HPLC
Entrez Gene Number

• NM_002658.2

Entrez Gene Summary This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer disease and also with decreased affinity for fibrin-binding. The protein encoded by this gene converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. This gene's proprotein is cleaved at a Lys-Ile bond by plasmin to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor.
Gene Symbol

• PLAU
• UPA
• uPA
• URK
• u-PA
• ATF
• EC 3.4.21.73 [Contains: Urokinase-type plasminogen activator long chain A
• Urokinase-type plasminogen activator short chain A
• Urokinase-type plasminogen activator chain B].

UniProt Number

• P00749

UniProt Summary FUNCTION: SwissProt: P00749 # Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
SIZE: 431 amino acids; 48525 Da
SUBUNIT: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high molecular mass form contains a long chain A which is cleaved to yield a short chain A. Binds LRP1B; binding is followed by internalization and degradation. Interacts with MRC2. Interacts with PLAUR.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in the prostate gland and prostate cancers.
PTM: Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
SIMILARITY: SwissProt: P00749 ## Belongs to the peptidase S1 family. & Contains 1 EGF-like domain. & Contains 1 kringle domain. & Contains 1 peptidase S1 domain.
Usage Statement

• Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Conditions Store at -20°C. This product is stable for 3 year as supplied
Reconstitute with H(2)O, Following reconstitution, store at 4°C, stock solution is stable for 1-2 months at 4°C. Avoid exposure to light; material is hydroscopic
Material Size 5 mg