Merck - C6219-100UL - Rabbit Anti-connexin 43 100ul

ITEM #: 13017619
The 43 kDa conne x in protein (conne x in 43, C x 43) is a phosphoprotein that is e x pressed in most tissues, even though the pattern of e x pression may differ in various cell types (e.g., in the brain, it is found in astrocytes, ependyma and leptomeninges, but not in neurons, oligodendrocytes and pinealocytes; or in the liver, it is present in Ito cells, but not in hepatocytes). Gap junction protein levels change in response to disruption of tissue architecture. For instance, an increased e x pression of C x 43 was found in early stages of atherosclerosis.
Synonyms: Conne x in 43 Antibody; Conne x in 43 Antibody - Anti-Conne x in-43 antibody produced in rabbit; C x 43 Antibody
MDL Number: MFCD00803532
Storage: -20C
Application: Anti-Conne x in-43 may be used in immunoblotting, immunocytochemistry and immunohistochemistry (frozen and formalin-fi x ed, paraffin-embedded tissues). Polyclonal antibodies reacting specifically with C x 43 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties.A minimum working dilution of 1:8, 000 is determined by immunoblotting using a whole e x tract from mouse brain.A minimum working dilution of 1:400 is determined by indirect immunofluorescent staining of acetone-fi x ed cultured baby hamster kidney (BHK).A minimum working dilution of 1:2, 000 is determined by indirect immunofluorescent staining of rat heart. (Negative on rat liver sections)A minimum working dilution of 1:2, 000 is determined by indirect immunopero x idase staining of trypsin-digested, formalin-fi x ed, paraffin-embedded human or animal tissue.
Biochem Physiol Actions: Conne x in-43 (C x -43) might play a vital role in neuronal migration as it resides in radial glial fibers which are closely associated with migrating neurons. It is also involved in transmembrane NAD+ transport. Conne x in-43, which is an element of gap junction, facilitates cell-cell communication. C x -43 is known to induce [Ca2+]i burst phenomenon in uterine artery endothelial cells (UAECs). Elevated e x pression of C x -43 has been observed during early stages of human coronary atherosclerosis. Reduction or alteration in the levels or types of conne x in e x pressed in various cell types correlates with tumor progression and metastasis. However, glioma cells transfected with the oncogene neu (c-erb-B2) e x hibit a major reduction in intercellular communication with no decrease in overall e x pression of C x 43. Different states of rat C x 43 phosphorylation are found during development and lactation. Conne x in 43 mutations occur in children whose hearts failed to develop asymmetry and lethal heart malformations occur on knocking out this gene in mice. Polyclonal antibodies reacting specifically with C x 438-11 may be applied in diverse cellular and molecular approaches to the study of gap junctions and their properties, and to correlate their e x pression pattern in a variety of cell types and tissues with physiological functions or pathological conditions.
RABBIT ANTI-CONNEXIN 43 100UL
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