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Merck - CBA059-1KIT - Annexin V-FITC Apoptosis Detection Kit II

  • MFR SKU: CBA059-1KIT
  • ITEM #: 6312228
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ITEM #: 6312228
Annexin V-FITC Apoptosis Detection Kit II^ Annexin V-FITC Apoptosis Detection Kit II, Kit contains: Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol, Format: Flow cytometry or fluorescence microscopy, Form 20 Tests, Detection method: Fluorescence.
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Merck - CBA059-1KIT - Annexin V-FITC Apoptosis Detection Kit II

  • Item #: 6312228
  • MFR SKU: CBA059-1KIT
Technical Specs
  • Overview A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2+ , Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.
  • Catalogue Number CBA059
  • Brand Family Calbiochem®
  • Materials Required but Not Delivered • 1X PBS
  • • Flow cytometer or fluorescence microscope
  • References Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
  • Frey, T. 1997. Cytometry 28, 253;
  • Boersma, A.W.M., et al. 1996. Cytometry 24, 123.
  • White, E. 1996. Genes and Devel. 10, 1.
  • Martin, S.J., et al. 1995. J. Exp. Med. 182, 1545.
  • Koopman, G., et al. 1994. Blood 84, 1415.
  • Wyllie, A.H., 1993. Br. J. Cancer 67, 205.
  • Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
  • Fadok, V.A., et al. 1992. J. Immunology 148, 2207.
  • Schmid, I., et al. 1992. Cytometry 13, 204.
  • Kerr, J.F.R., et al. 1972. Cancer 26, 239.
  • Detection method Fluorescence
  • Form 20 Tests
  • Format Flow cytometry or fluorescence microscopy
  • Kit contains Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
  • Quality Level MQ100
  • Key Applications Flow Cytometry
  • Immunofluorescence
  • Assay time 30 min
  • Sample Type Intact cells
  • Intended use The Calbiochem ® Annexin V-FITC Apoptosis Detection Kit II is a non-isotopic system that can be used to detect phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using flow cytometry or fluorescence microscopy.
  • Ship Code Blue Ice Only
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage +2° C to +8° C
  • Storage Conditions Upon arrival store the entire contents of the kit at 4° C.
  • Do not freeze Ok to freeze
  • Kit contains Annexin V-FITC, Binding Buffer, Propidium Iodide and a user protocol.
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