Merck - DAB500 - DAB500, IHC Select HRP/DAB, 150 Test IHC Select HRP/DAB Tests for Immunocytochemistry, Immunohistochemistry & Immunohistochemistry (Paraffin).

ITEM #: 11559206
Summary and E x planation: Immunohistochemical techniques have been in use as adjunctive methods for the recognition of cells in tissue sections for at least 50 years. The first method reported use of fluorescent labels in 19421. In 1974, reports of using enzymatic labels, such as horseradish pero x idase (HRP) in routine H & E paraffin sections appeared2, 3. The methods have since become the ′standard of care′ in surgical pathology when classic methods alone fail to yield a definitive diagnosis4-9. Early techniques were based on the pero x idase-anti-pero x idase (PAP) reaction28, while improvements e x ploited the strong affinity of avidin for biotin10. This technique uses HRP-labeled streptavidin. The secondary antibody is conjugated with biotin and the streptavidin-HRP comple x reacts with the biotin on the secondary antibodies. The resulting biotin-avidin-HRP comple x can react with the primary antibody bound to the specific epitope of the target antigen. The HRP enzymes of the streptavidin comple x then catalyze the substrate/chromogen reaction to form a colored reaction product (brown to black when using DAB as the chromogen) at the antigen site. A biological stain is then used to visualize the whole tissue section.
Application: The IHC Select Immunopero x idase Secondary Detection System is intended for use with the CHEMICON IHC Select Prediluted Primary Antibody reagents which contain rabbit and mouse IgG for the qualitative identification of antigens by light microscopy in paraffin-embedded tissues. The IHC Select Immunopero x idase Secondary Detection System may also be used with other rabbit or mouse IgG primary antibodies. General Purpose Reagents
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DAB500, IHC Select HRP/DAB, 150 Test IHC Select HRP/DAB Tests for Immunocytochemistry, Immunohistochemistry & Immunohistochemistry (Paraffin).
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