Merck - ECM630 - ECM630, Fibrin In Vitro Angiogenesis Assay The Fibrin Gel In Vitro Angiogenesis Assay Kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored.

ITEM #: 11559320
Introduction Angiogenesis is the process of generating new capillary blood vessels. It is a fundamental component of a number of normal (reproduction and wound healing) and pathological processes (diabetic retinopathy, rheumatoid arthritis, tumor growth and metastasis)1. The CHEMICON Fibrin Gel In Vitro Angiogenesis Assay Kit provides a convenient system for evaluation of tube formation by endothelial cells in 96-well or other formats. When cultured on top or within a fibrin gel, endothelial cells rapidly align and form interconnecting networks that can display patent lumina2, 3. Tube formation is a multi-step process involving cell adhesion, migration, differentiation and growth4. The formation of intercellular connections and lumina within endothelial cell networks in fibrin gels is dependent upon the actions of VE-cadherin, avb3 and a5b1 integrins, the cdc42 and Rac1 GTPases, and membrane-type matri x metalloproteinases (MT-MMPs)2, 3, 5, 6. Angiogenesis within fibrin gels in vitro is regarded as an accurate model for wound healing and tumor angiogenesis, as tumor cell-derived vascular endothelial growth factor/vascular permeability factor promotes leakage of fibrinogen from the tumor vasculature and formation of a fibrin-rich proangiogenic provisional matri x 7. Fibrin gel formation is initiated by enzymatic cleavage of fibrinogen, a heterotrimer, by thrombin8. The resulting cleaved fibrin molecules form regular, multimolecular arrays that are highly translucent. The concentrations and formulations of the fibrinogen and thrombin in this kit are optimized for ma x imal tube-formation by HUVEC and easy visualization of these tubes.
Application: Research CategoryCell Structure
: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
ECM630, Fibrin In Vitro Angiogenesis Assay The Fibrin Gel In Vitro Angiogenesis Assay Kit represents a simple model of angiogenesis in which the induction or inhibition of tube formation by exogenous signals can be easily monitored.
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