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Merck - HCS30-1EA - BrdU Immunohistochemistry System, 1EA

  • MFR SKU: HCS30-1EA
  • ITEM #: 3450422
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ITEM #: 3450422
BrdU Immunohistochemistry System^ Kit contains: Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated mouse anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides.
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Merck - HCS30-1EA - BrdU Immunohistochemistry System, 1EA

  • Item #: 3450422
  • MFR SKU: HCS30-1EA
Technical Specs
  • Overview Useful for the detection of proliferating cells within fixed tissue samples or cell populations. Requires metabolic incorporation of BrdU (Cat. No. 203806 ) into sample prior to paraffin-embedding.
  • Catalogue Number HCS30
  • Brand Family Calbiochem®
  • Materials Required but Not Delivered • Phosphate buffered saline (PBS)
  • • Quenching solution for endogenous peroxidase
  • • Alcohol and xylene
  • • Coverslips
  • • BrdU (i.e. Calbiochem Cat. No. 203806)
  • References Zhang, Q. and Paria, B.C. 2006. Endocrinology 147 , 2215.
  • Fukuda, K ., et al. 1990. Anal. Quant. Cytol. Histol. 12 , 135.
  • Cattoretti, G. et al. 1993. J. Pathol. 171 , 83.
  • Schutte, B., et al. 1987. J. Histochem. & Cytochem. 35 , 1343.
  • Gonchoroff , N.J., et al. 1986. J. Immunol. Meth. 93 , 97.
  • Ellwart, E. and Dormer, P. 1985. Cytometry 6 , 513.
  • Gratzner, H.G., et al. 1975. Expl. Cell Res. 95 , 88.
  • Detection method Microscopy
  • Form 50 Tests
  • Kit contains Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated sheep anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides (1 stained and 4 unstained), and a user protocol.
  • Positive control Any cell or tissue sample labeled with BrdU
  • Assay time ~2.5 hours plus labeling time
  • Sample Type Paraffin-embedded tissues or fixed cells
  • R Phrase R: 25-36/37/38-42/43-61
  • Toxic if swallowed.
  • Irritating to eyes, respiratory system and skin.
  • May cause sensitization by inhalation and skin contact.
  • May cause harm to the unborn child.
  • S Phrase S: 24-26-36/37/39-45
  • Avoid contact with skin.
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Wear suitable protective clothing, gloves and eye/face protection.
  • In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
  • Ship Code Dry Ice Only
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage -20° C
  • Storage Conditions Upon arrival store the entire contents of the kit at -20° C. Avoid contact with DAB; wearing of latex or rubber gloves is recommended.
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
  • Kit contains Trypsin concentrate, trypsin diluent, denaturing solution, blocking solution, biotinylated sheep anti-BrdU, streptavidin-peroxidase, substrate buffer reaction mix, DAB concentrate, hematoxylin, mounting media, control slides (1 stained and 4 unstained), and a user protocol.
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