Merck - MAK307-1KT - Malachite Green Phosphate Assay Kit; sufficient for 2500 colorimetric tests

ITEM #: 14505432
Features and Benefits
Compatible with high-throughput handling systems.
Application
Malachite Green Phosphate Assay Kit has been used to measure the liberation of free orthophosphate in the following applications:

• Phosphatase Assays: liberation of phosphate from peptide, protein, or small molecule substrate
• Lipase Assays: liberation of phosphate from phospholipids
• Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc).
• Quantitation of phosphate in phospholipids, proteins and DNAs, etc.
• Drug Discovery: high-throughput screen for phosphatase inhibitors.

Malachite green phosphate assay kit has been used in phosphate quantification and steady-state kinetics
General description
Malachite Green is a basic dye belonging to the triphenylmethane group. It is used in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate. [1] [2] The Malachite Green Phosphate Assay Kit is based on quantification of the green comple x formed between Malachite Green, molybdate and free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600 - 660 nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be e x ecuted in tubes, cuvettes or multi-well plates. The assays can be conveniently performed in 96 and 384 well plates for high throughput screening of enzyme inhibitors.
Suitability
Suitable for phosphate determination in a variety of biological and environmental samples.
Malachite Green Phosphate Assay Kit; sufficient for 2500 colorimetric tests
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