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Merck - PF060-.155ML - TDT ENZYME-.155ML 0.155ML (Each)

  • MFR SKU: PF060-.155ML
  • ITEM #: 3449976
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ITEM #: 3449976
Terminal Deoxynucleotidyl Transferase (TdT)^ Biological Activity: Contains 775 units of TdT. One unit is the amount of enzyme required to transfer 1.0nmol of dAMP from dATP to the 3' -OH terminus of d(A)50 in 60 minutes at 37[degree]C. Application: DNA labeling. 0.155mL liquid.
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Merck - PF060-.155ML - TDT ENZYME-.155ML 0.155ML (Each)

  • Item #: 3449976
  • MFR SKU: PF060-.155ML
  • Overview Native TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxy terminus of either double of single stranded DNA in a template-independent manner.
  • Catalogue Number PF060
  • Brand Family Calbiochem®
  • Synonyms Terminal Deoxynucleotidyl Transferase
  • References Chang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem. 21, 27.
  • Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
  • Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem. 257, 14773.
  • Roychoudhury, R. and R. Wu. 1980. Methods Enzymol. 65, 43.
  • Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
  • Roychoudhury, R., et al. 1976. Nucleic Acids Res. 3, 101.
  • Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.
  • Unit of Definition One unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3-OH terminus of d(A)50 in 60 min at 37° C.
  • Form Liquid
  • Formulation 775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.
  • Preservative None
  • Quality Level MQ100
  • Key Applications DNA Labeling
  • Application Notes DNA Labeling
  • Ship Code Dry Ice Only
  • Toxicity Standard Handling
  • Storage -20° C
  • Avoid freeze/thaw Avoid freeze/thaw
  • Do not freeze Ok to freeze
  • Special Instructions Following initial thaw, aliquot and freeze (-20° C).

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