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Merck - QIA76-100TEST - LIVE/DEAD DOUBLE STAINING KIT (Each)

  • MFR SKU: QIA76-100TEST
  • ITEM #: 6312576
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ITEM #: 6312576
Live/Dead Double Staining Kit^ Live/Dead Double Staining Kit, Kit contain: Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol. Format: Fluorescence microscopy, Species reactivity: a broad range of species, Detection method: Fluorescence, Sample type Cell suspensions.
This listing is for Each

Merck - QIA76-100TEST - LIVE/DEAD DOUBLE STAINING KIT (Each)

  • Item #: 6312576
  • MFR SKU: QIA76-100TEST
Technical Specs
  • Overview This kit uses a cell-permeable green fluorescent Cyto-dye (Ex. max.: 488 nm; Em. max.: 518 nm) to stain live cells, and propidium iodide (Ex. max.: 488 nm; Em. max.: 615 nm) to stain dead cells. Stained live and dead cells can be visualized by fluorescence microscopy using a band-pass filter which detects FITC and rhodamine. Viable cells will stain only with the Cyto-dye, fluorescing green, whereas the dead cells will stain with both Cyto-dye (green) and propidium iodide (red), resulting in a yellow fluorescence. Note: 1 T = 1 test.
  • Optimal staining conditions may vary among different cell types and should be determined empirically by the investigator.
  • Catalogue Number QIA76
  • Brand Family Calbiochem®
  • Materials Required but Not Delivered • Fluorescent microscope with a band-pass filter that detects FITC and rhodamine
  • • Microscope slides and cover slips
  • References Luther, E., and Kamenstsky, L.A. 1996. Cytometry 23, 272.
  • Frey. T., et al. 1995. Cytometry 21, 265.
  • Detection method Fluorescence
  • Form 100 Tests
  • Format Fluorescence microscopy
  • Kit contains Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
  • Quality Level MQ100
  • Assay time 0.5 h
  • Sample Type Cell suspensions or adherent cells
  • Species Reactivity
  • A Broad Range Of Species
  • R Phrase R: 36/37/38-46
  • Irritating to eyes, respiratory system and skin.
  • May cause heritable genetic damage.
  • S Phrase S: 26-36/37/39-45
  • In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Wear suitable protective clothing, gloves and eye/face protection.
  • In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
  • Intended use The Calbiochem ® Live/Dead Double Staining Kit can be used to distinguish between live and dead cells, which is essential for the study of growth control and cell death.
  • Ship Code Dry Ice Only
  • Toxicity Multiple Toxicity Values, refer to MSDS
  • Storage -20° C
  • Storage Conditions Upon arrival, store the entire contents of the kit at -20° C. Following initial use of the Staining Buffer, refrigerate (4° C) the remaining buffer.
  • Protect from Moisture Protect from moisture
  • Do not freeze Ok to freeze
  • Kit contains Staining Buffer, Cyto-Dye, Propidium Iodide, and a user protocol.
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