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Merck - SCR028 - Mesenchymal Stem Cell Osteogenesis Kit

  • MFR SKU: SCR028
  • ITEM #: 11569656
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$1,548.50
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ITEM #: 11569656
The Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining.

Merck - SCR028 - Mesenchymal Stem Cell Osteogenesis Kit

  • Item #: 11569656
  • MFR SKU: SCR028
  • 50% mature osteocytes from rat bone marrow derived mesenchymal stem cells. Efficiency of osteogenic differentiation may vary, depending upon the quality of the mesenchymal stem cells and if variations to the protocol are introduced.
  • For Research Use Only; not for use in diagnostic procedure.
  • Materials Required but Not Delivered 1. Human or rat mesenchymal stem cell and cells culture reagents
  • 2. Mesenchymal Stem Cell Expansion Media (DMEM-low glucose, without glutamine), 10% heat-inactivated fetal bovine serum (Catalog No. ES-009-D), 2 mM L-Glutamine (Catalog No. TMS-002-C) and 1X solution of penicillin and streptomycin (Catalog No. TMS-AB2-C))
  • 3. 24-well tissue culture plates™
  • 4. Phosphate-Buffered Saline (1X PBS) (Catalog No. BSS-1005-B)
  • 5. Accutase TM (Catalog No. SCR005)
  • 6. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)
  • 7. Hemacytometer
  • 8. Microscope
  • Components
  • Dexamethasone Solution: (Catalog No. 90357) One vial containing 100 μ L of 10 mM dexamethasone in ethanol. Store at -20° C.
  • Ascorbic Acid 2-Phosphate Solution: (Catalog No. 2004011) One vial containing 500 μ L of 100 μ M ascorbic acid 2-phosphate in water. Store at -20° C.
  • Glycerol 2- Phosphate Solution: (Catalog No. 2004010) Three vials containing 1 mL of 1 M glycerol 2-phosphate in water. Store at -20° C.
  • Collagen, Type I: (Catalog No. 2004013) One vial containing 150 μg collagen Type I. Store at -20° C.
  • Vitronectin: (Catalog No. 2004012) One vial containing 150 μg vitronectin. Store at -20° C.
  • Alizarin Red Solution: (Catalog No. 2003999) One bottle containing 50 mL Alizarin Red Solution. Store at 2° to 8° C.
  • Detection method Colorimetric
  • Quality Level MQ100
  • Application The Mesenchymal Stem Cell Osteogenesis Kit contains all the reagents necessary to readily differentiate mesenchymal stem cells to an osteogenic lineage as assessed by Alizarin Red staining.
  • Stem Cell Type
  • Mesenchymal Stem Cells
  • Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • Storage Conditions Note: Kit components require two different storage temperatures.
  • Dexamethasone Solution, Ascorbic Acid 2-Phosphate Solution, Glycerol 2- Phosphate Solution, Collagen Type I, and Vitronectin should be stored at -20º C. Alizarin Red Solution should be stored at 2° to 8° C.
  • Precautions
  • · Dexamethasone is an irritant and potentially toxic. DMSO is readily absorbed through the skin. Wear a lab coat and gloves when handling these solutions.
  • Please refer to the Material Safety Data Sheet at for further precautions.
  • Material Size 1 kit

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