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Merck - WB59-100ML - WESTERN-RE-PROBE(TM)-100ML (Each)

  • MFR SKU: WB59-100ML
  • ITEM #: 6312656
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ITEM #: 6312656
Western-Re-Probe Reagent 100ml^ Form: Liquid, Formulationl: Supplied as a 5X solution, Application: Immunoblotting (Western Blotting), Kit contains: 5X Re-Probe solution (125 ml), Storage: +2 degree C to +8 degree C.
This listing is for Each

Merck - WB59-100ML - WESTERN-RE-PROBE(TM)-100ML (Each)

  • Item #: 6312656
  • MFR SKU: WB59-100ML
Technical Specs
  • Overview Designed to dissociate and remove antibodies from membrane-bound proteins without destroying antigenic binding capacity. Recommended for stripping chemiluminescent or radioisotopic signals from blots. Not recommended for stripping color-producing Western blots. Supplied as a 5X solution sufficient for stripping 25-30 standard blots.
  • A special formulation designed to dissociate and remove antibodies from the membrane bound protein without destroying the antigenic binding affinity.
  • Catalogue Number WB59
  • Brand Family Calbiochem®
  • Form Liquid
  • Formulation Supplied as a 5X solution.
  • Kit contains 5X Re-Probe solution (125 ml)
  • Quality Level MQ100
  • Key Applications Immunoblotting (Western Blotting)
  • Application Notes Immunoblotting (see comments)
  • Application Comments Sufficient volume to strip 25-30 standard blots.
  • ADVANTAGES
  • -Immunoblotting analyzed repeatedly with multiple probes
  • -Saving on hard-to-get reagents and samples
  • -Correction and re-analysis of unsatisfactory Western Blots
  • -Saving time spent in gel re-running and transfer processes
  • PROTOCOL
  • 1- Keep the membrane wet in an appropriate buffer after each probing procedure. Prepare 20-25 ml solution of 1X Western-Re-Probe™ in water.
  • 2- Incubate the membrane in 1X Western-Re-Probe™ buffer for 30 min at room temperature (RT) with shaking.
  • 3- Wash the membrane three times in PBST or TBST buffer containing 0.1% Tween ® -20 detergent. Use a large volume of washing buffer in a tray for the washing step. Each wash cycle involves agitating the membrane 5 min in the washing buffer.
  • 4- Block the membrane and perform immunodetection.
  • Western- Re -Probe™ Reagent can be used for stripping and re-probing membranes 2-3 times. Some loss of signal may be noticed after second cycle of stripping and re-probing.
  • PROTOCOL SUMMARY
  • 1. Incubate membrane in 1X Western-Re-Probe™ (30 min at RT) with shaking
  • 2. Wash the membrane 3 times in PBST or TBST buffer
  • 3. Block membrane and perform immunodetection
  • Ship Code Blue Ice Only
  • Toxicity Standard Handling
  • Storage +2° C to +8° C
  • Do not freeze Ok to freeze
  • Kit contains 5X Re-Probe solution (125 ml)
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