Minerva Biolabs - 11-9100 - VenorGeM qEP, 100 Tests

ITEM #: 13103411
VenorGeM qEP utilizes quantitative, real-time PCR (qPCR). The kit can be performed with any type of real-time PCR cycler able to detect the fluorescence dyes FAM and HEX. The protocol provided is preferred for fast and reliable screening of cell culture supernatants most applicable in research and development. The detection procedure can be performed within 3 hours.
Mycoplasmas are specifically detected by amplifying a highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. The mycoplasma-specific amplification is detected at 520 nm (FAM channel).The kit includes primer and FAM labeled probes which allow the specific detection of all Mollicutes species so far described as contaminants of cell cultures and media components. Eukaryotic DNA is not amplified by this primer/probe system.
False negative results due to PCR inhibitors or improper DNA e x traction are detected by the internal amplification control which can be added to the PCR master mi x . The amplification of the internal amplification control is detected at 610 nm (HEX channel).
Type of PCR
TaqMan based qPCR Assay with FAM and HEX labeled probes
Recommended Use / Scope
VenorGeM qEP is used for direct detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) contamination in cell cultures and cell media components.
Kit Components

• lyophilized primer/nucleotide/probe/polymerase/mi x in aliquots of 25 tests
• rehydration buffer
• lyophilized internal amplification control
• lyophilized positive control
• PCR grade water

Result Evaluation
cycler based, real-time PCR
Required Consumables
PCR reaction tubes
Optional for process validation and EP 2.6.7 compliant testing:
Internal Control DNA e x tra (4 vials for 300 µl each of internal amplification control; Cat. No. 11-9905)
10CFU Sensitivity Standards available for all EP 2.6.7 listed mycoplasma species
Required Lab Devices

• qPCR cycler with FAM and HEX filter
• variable microliter pipettes
• benchtop centrifuge for 1.5 ml reaction tubes

Shelf Life and Storage
Components can be stored at +2 to +8°C for at least 12 months. After rehydration the reagents must be stored at -18°C.
EP 2.6.7 Compliance
Yes.
VenorGeM qEP, 100 Tests
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