MP Biomedicals - 0219530305 - Lysozyme Type VI from Chicken Eggwhite, 5g

ITEM #: 13275845
Lysozyme (muramidase) hydrolyzes preferentially the b-1, 4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.
Lysozyme has been used for lysing E. coli and Streptomycetes for e x traction purposes such as e x tracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitode x trin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, ma x imal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25°C using Micrococcus lysodeikticus as substrate.
Pkg. Type : Amber Glass Bottle
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