MP Biomedicals - 11S7100KIT - PEROXIDASE DETCN KIT LABEL APOPTAG 40ASY (Each)

ITEM #: 12325178
ApopTag Pero x idase in situ detection kits label apoptotic cells in research samples by modifying DNA fragments utilizing terminal deo x ynucleotidyl transferase (TdT) for detection of apoptotic cells by immuno-pero x idase detection of digo x igenin-labeled genomic DNA in thin sections of fi x ed tissue.
ApopTag Pero x idase in situ detection kits stains DNA strand breaks which in turn is detected by the TUNEL assay and subsequently visualised by light microscopy.
TUNEL kit for use with light microscopy ApopTag Pero x idase in situ Detection Kits detect apoptotic cells by immuno-pero x idase detection of digo x igenin-labeled genomic DNA in thin sections of fi x ed tissue. The ApopTag Pero x idase Plus kit includes positive control slides to aid identification of apoptotic cells. Principles of the procedure: Apoptotic cells are detected in situ by specific end labeling and detection of the DNA fragments produced by the apoptotic process. Residues of digo x igenin-nucleotide are catalytically added to the DNA by terminal deo x yribonucleotidyl transferase (TdT), an enzyme which catalyzes a template-independent addition of deo x yribonucleotide triphosphate to the 3’-OH ends of double- or single-stranded DNA. The incorporated nucleotides form a random heteropolymer of digo x igenin-11-dUTP and dATP, in a ratio that has been optimized for antidigo x igenin antibody binding. The anti-digo x igenin antibody fragment carries a conjugated reporter enzyme (pero x idase) to the reaction site. The localized pero x idase enzyme then catalytically generates an intense signal from a chromogenic substrate. This system allows for sensitive and specific staining of the very high concentration of 3’-OH ends that are localized in apoptotic bodies.
Principles of the procedure: Apoptotic cells are detected in situ by specific end labelling and detection of the DNA fragments produced by the apoptotic process. Residues of digo x igenin-nucleotide are catalytically added to the DNA by terminal deo x yribonucleotidyl transferase (TdT), an enzyme which catalyzes a template-independent addition of deo x yribonucleotide triphosphate to the 3'-OH ends of double or single-stranded DNA. The incoorporated nucleotides form a random heteropolymer of digo x igenin-11-dUTP and dATP, in a ratio that has been optimized for antidigo x igenin antibody binding. The anti-digo x igenin antibody fragment carries a conjugated reporter enzyme (pero x idase) to the reaction site. The localized pero x idase enzyme then catalytically generates an intense signal from a chromogenic substrate. This system allows for sensitive and specific staining of the very high concentration of 3'-OH ends that are localized in apoptotic bodies.
ApopTag Peroxidase, 40 Assays
This listing is for Each