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Omega Bio-Tek - D6915-03 - E.Z.N.A. Endo-Free Plasmid DNA Midi Kit - 25 Preps

  • MFR SKU: D6915-03
  • ITEM #: 3327135
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ITEM #: 3327135
The E.Z.N.A. Endo-Free Plasmid DNA Midi Kit provides an efficient method for the large-scale isolation of low-endoto x in plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endoto x ins are removed, and the protocol follows a simple bind-wash-elute procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions....
ITEM #: 3327135
The E.Z.N.A. Endo-Free Plasmid DNA Midi Kit provides an efficient method for the large-scale isolation of low-endoto x in plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endoto x ins are removed, and the protocol follows a simple bind-wash-elute procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 µg of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for sensitive downstream applications such as transfection of mammalian cells, cloning, automated fluorescent DNA sequencing (typical reads e x ceed 800 bp), ne x t-generation sequencing, restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, etc.
E.Z.N.A. Endo-Free Plasmid DNA Midi Kit - 25 Preps
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Omega Bio-Tek - D6915-03 - E.Z.N.A. Endo-Free Plasmid DNA Midi Kit - 25 Preps

  • Item #: 3327135
  • MFR SKU: D6915-03
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