OZ Biosciences - MRNA32-20 - Cre Mrna: Cre Recombinase Encoding Mrna 20g

ITEM #: 17057870
Wild-type Cas9 mRNA is a stabilized non-immunogenic messenger RNA (mRNA) that has been designed to produce high e x pression level of wild-type Cas9 protein. It creates a double stranded break at a target site delineated by RNA guide sequences. This mRNA is produced by in vitro transcription, stabilized at the 5 end by modified nucleotides capping and contain a poly(A) tail at the 3 end. mRNACas9 is optimized to yield improved stability and performance and to lower the cellular innate immunity response.
Plasmids and viral vectors are traditionally used in CRISPR genome editing to e x press the required proteins. However, there is a risk to use DNA instead of mRNA: Double stranded DNA breaks catalyze insertion of DNA at the cut site. At some substantial frequency, the protein e x pression vectors can integrate, which can lead to continuous e x pression of the nuclease or a previously silent sequence.
NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localization sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyzes recombination between two lo x P sites.
This mRNA is produced by in vitro transcription, stabilized at the 5 end by modified nucleotides capping and contain a poly(A) tail at the 3 end. CRE mRNA is optimized to yield improved stability and performance and to lower the cellular innate immunity response. Plasmids and viral vectors are traditionally used in CRISPR genome editing to e x press the required proteins. However, there is a risk to use DNA instead of mRNA: Double stranded DNA breaks catalyze insertion of DNA at the cut site. At some substantial frequency, the protein e x pression vectors can integrate, which can lead to continuous e x pression of the nuclease or a previously silent sequence.
Cre Mrna: Cre Recombinase Encoding Mrna 20g
This listing is for Each