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Quality Biological - 118-093-721EA - TRYPSIN 0.25% EDTA 0.02% 100ML (Each)

  • MFR SKU: 118-093-721EA
  • ITEM #: 4053230
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ITEM #: 4053230
Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface.
Applications
Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carbo x yl sides of Lysine and Arginine, e x cept when either...
ITEM #: 4053230
Trypsin is a proteolytic enzyme used to detach adherent cell from culture vessel surfaces. Typical use includes removing adherent cells from a culture surface.
Applications
Trypsin is a serine protease derived from porcinepancreas. It is a single chain polypeptide of 223 amino acid residue with substrate specificity based on positively charged Lysine and Arginine side chains. Trypsin predominantly cleaves peptide chains at the carbo x yl sides of Lysine and Arginine, e x cept when either is followed by Proline. It is most commonly used for dissociation and disaggregation of adherent cells.
Ethylenediaminetetraacetic acid (EDTA), a chelating agent often added to enhance enzymatic activity of trypsin solution. EDTA acts by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion. Polyvinylpyrrolidone (PVP) a synthetic polymer is known to increase cell viability especially in low serum or serum free condition.
Trypsin, 0.05% - EDTA, 0.1% assists in the dissociation of cell monolayers and tissue; most useful when dissociating fibroblasts and keratinocytes. While, Trypsin, 0.25% - EDTA, 0.02%, assists in detaching normal and transformed cells from vessel surfaces.
Trypsin 0.25% EDTA 0.02%, 100mL
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Quality Biological - 118-093-721EA - TRYPSIN 0.25% EDTA 0.02% 100ML (Each)

  • Item #: 4053230
  • MFR SKU: 118-093-721EA
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