Roche Diagnostic - 11855476001 - Titan One Tube RT-PCR System

ITEM #: 13106719
Storage: -20C
UNSPSC Code: 41106300
General description: The Titan One Tube RT-PCR System uses Reverse Transcriptase AMV for first strand cDNA synthesis and the E x pand High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by PCR. The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:. Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.. Delivers increased sensitivity due to the high efficiency of all three enzymes.. Is faster than conventional two-step RT-PCR.
Application: . The one-tube reaction system will reverse transcribe RNA into cDNA with Reverse Transcriptase AMV, then amplify the cDNA with a thermal cycling reaction using the optimized enzyme blend from the E x pand High Fidelity PCR System. Depending on the primers used for the RT-PCR techniques, a specific message or the entire population of transcripts can be amplified. It can be also used for:Determining the presence or absence of RNA templates. Cloning of rare messages without the need to construct cDNA libraries. Quantifying the levels of gene e x pression
Specificity: Specificity of the reaction strongly depends on primer design and DNA e x clude artefacts from DNA targets appropriate positive and negative control reaction should be inactivation: 2 min at 94 CReverse Transcriptase AMV
Other Notes: For life science research only. Not for use in diagnostic procedures.
Titan One Tube RT-PCR System
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