Rockland Immunochemical - 88-1488-31 - Goat TrueBlot Set (with IP Agarose beads), 1Set, Liquid (sterile filtered)

ITEM #: 6153419
Goat IgG True. Blot is provided as 1000X solution. To conserve reagent, we recommend incubating the blots from minigels in sealed bags (removing as much air as possible) with minimal volume (2-3 m. Ls). If used conservatively at 2.5mls/blot will yield enough reagent for 20 blots. Note that there are three key procedural considerations: 1. Protein A or G beads may be used with the mouse, goat and sheep True. Blot secondaries, but not with the rabbit True. Blot secondary. Use of protein A or G beads with the rabbit True. Blot will result in contaminating bands. 2. Immunoprecipitate should be completely reduced. 3. BLOTTO/Milk should be used as the blocking protein for the immunoblot. MB-70 or BSA is not an effective blocker. Goat True. Blot Set Components: 1. Goat IgG True. Blot HRP-conjugated monoclonal secondary antibody reacting with goat Ig. Gs for optimal signal detection in immunoprecipitation/immunoblotting e x periments. 2. Anti-Goat Ig IP Beads: 2.5 mL. Binds 1 mg Ig/mL beads. 3. Western blot incubation tray. Special Notes: Upon initial use of the IP beads, we recommend that the vial be inverted several times to get the beads into suspension. We recommend using a large bore pipet to pipet up the liquid for use. For storage of the opened vial of beads, we recommend that the vial cap be sealed with parafilm to help prevent evaporation of the buffer. All recommended dilutions for listed applications are intended as an initial recommendation, specific conditions for each protein and antibody combination should be specifically optimized by the end user.
Goat True. Blot Set (with IP Agarose beads), 1Set, Liquid (sterile filtered)
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