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Zymo Research - F9001-5 - 5-FLUOROOROTIC ACID (5-FOA) >/-98 (Each)

  • MFR SKU: F9001-5
  • ITEM #: 3298049
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ITEM #: 3298049
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nonto x ic to yeast, 5-FOA is converted to the to x ic form (i.e., 5-flurouracil) in strains e x pressing the functional URA3 gene coding for orotine-5'-monophosphate decarbo x ylase that is involved in the synthesis of uracil. Yeast strains...
ITEM #: 3298049
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nonto x ic to yeast, 5-FOA is converted to the to x ic form (i.e., 5-flurouracil) in strains e x pressing the functional URA3 gene coding for orotine-5'-monophosphate decarbo x ylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection.
The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and a 2X concentrated (2 mg/ml) synthetic complete 5-FOA (i.e., 2X SC/5-FOA) in liquid medium containing yeast nitrogen base, ammonium sulfate, complete amino acids, and glucose. Both have been tested and validated on the basis of counter selection activity.
5-Fluoroorotic Acid (powder) (5 g)
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Zymo Research - F9001-5 - 5-FLUOROOROTIC ACID (5-FOA) >/-98 (Each)

  • Item #: 3298049
  • MFR SKU: F9001-5
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